PP – 39 The Cytotoxic Effects of Turmeric-Black Pepper-Cinnamon Extracts Mix on Breast Cancer Cell Line MDA-MB-231

Duygu AYGÜNEŞ1, Canfeza SEZGİN2, Buket KOSOVA CAN1

  1. Ege University Medical Faculty Department of Medical Biology
  2. Ege University Medical Faculty Department of Medical Oncology

In recent years, herbal therapies have been widely used to support the effects of chemicals applied in conventional cancer treatment. Aromatic plants are the source of many drugs used in cancer treatment and the most important ones for support therapy options. In the literature, there are various studies indicating that turmeric 1 (Curcuma longa), black pepper 2 (Piper nigrum), and cinnamon 3 (Cinnamomum sp.) extracts have anti-bacterial, anti-inflammatory, anti-viral, anti-oxidant and anticancer activity.

In our study, we aimed to investigate the cytotoxic effects of a mixture of extracts obtained from these three plants, on human breast cancer cell line MDA-MB-231.

Turmeric-Black Pepper-Cinnamon extract mixture (ZKT) was supplied by FGC Consulting Natural and Herbal Products Ltd. Co. (Urla, Izmir, Turkey). WST-1 cell viability assay (Roche, Mannheim, Germany) was performed to determinate the cytotoxic effects of ZKT on breast cancer cells. The cells were plated at a cell density of 1 X 104 cells in 96-well plates and grown with Leibovitz’s L-15 Medium containing various concentrations (1 /10 -1/107) of ZKT, depending on the experiment. The cells were treated by different concentrations during 24, 48 and 72 hours. The absorbance values of WST-1 reagent added cells were measured at a microplate reader plate. GraphPAD 6.0 (La Jolla, California, USA) software was used. For graphs and statistical analysis.

After the evaluation of cytotoxicity assay results, it is determined that ZKT has a significant cytotoxic effect on breast cancer cell line MDA-MB-231. IC50 value for 24 hours was determined as 1/50 dilution ratio.

In our study, the cytotoxicity of the turmeric-black pepper-cinnamon extract mixture was determined using cell viability assays and it has been observed that components of ZKT may be promising in clinical breast cancer treatment as a support theraphy.

As continuation of our study, the ZKT active ingredients, turmeric extract curcumin and black papper extract piperine which improves the bioavailability of curcumin, will be encapsulated nanotechnologically. The cytotoxic activity on the breast cancer will be assessed as in vitro and in vivo with these nanoparticules and these experiments will continue by combining with the chemotherapeutics that used in breast cancer treatment.

Resources:

1) Lin et al. Drug Deliv. 2015, Jul 23:1-6.

2) Greenshields et al. Cancer Lett. 2015, Feb 1;357(1):129-40.

3) Lu et al. Carcinogenesis. 2010 Mar;31(3):481-8.